[Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose synthase].
Identifieur interne : 003031 ( Main/Exploration ); précédent : 003030; suivant : 003032[Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose synthase].
Auteurs : Z E Grushetskaia ; V A Lemesh ; L V KhotylevaSource :
- TSitologiia i genetika [ 0564-3783 ]
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Amorces ADN (génétique), Arabidopsis (enzymologie), Arabidopsis (génétique), Cellulose (biosynthèse), Cellulose (génétique), Données de séquences moléculaires (MeSH), Glucosyltransferases (génétique), Gènes de plante (MeSH), Lin (croissance et développement), Lin (enzymologie), Lin (génétique), Paroi cellulaire (génétique), Paroi cellulaire (métabolisme), Réaction de polymérisation en chaîne (MeSH), Séquence nucléotidique (MeSH), Étiquettes de séquences exprimées (MeSH).
- MESH :
- biosynthèse : Cellulose.
- croissance et développement : Lin.
- enzymologie : Arabidopsis, Lin.
- génétique : ADN complémentaire, Amorces ADN, Arabidopsis, Cellulose, Glucosyltransferases, Lin, Paroi cellulaire.
- métabolisme : Paroi cellulaire.
- Données de séquences moléculaires, Gènes de plante, Réaction de polymérisation en chaîne, Séquence nucléotidique, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Arabidopsis (enzymology), Arabidopsis (genetics), Base Sequence (MeSH), Cell Wall (genetics), Cell Wall (metabolism), Cellulose (biosynthesis), Cellulose (genetics), DNA Primers (genetics), DNA, Complementary (genetics), Expressed Sequence Tags (MeSH), Flax (enzymology), Flax (genetics), Flax (growth & development), Genes, Plant (MeSH), Glucosyltransferases (genetics), Molecular Sequence Data (MeSH), Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , biosynthesis : Cellulose.
- enzymology : Arabidopsis, Flax.
- genetics : Arabidopsis, Cell Wall, Cellulose, DNA Primers, DNA, Complementary, Flax, Glucosyltransferases.
- growth & development : Flax.
- metabolism : Cell Wall.
- Base Sequence, Expressed Sequence Tags, Genes, Plant, Molecular Sequence Data, Polymerase Chain Reaction.
Abstract
Cellulose synthase catalytic subunit genes, CesA, have been discovered in several higher plant species, and it has been shown that the CesA gene family has multiple members. HVR2 fragment of these genes determine the class specificity of the CESA protein and its participation in the primary or secondary cell wall synthesis. The aim of this study was development of specific and degenerated primers to flax CesA gene fragments leading to obtaining the class specific HVR2 region of the gene. Two pairs of specific primers to the certain fragments of CesA-1 and CesA-6 genes and one pair of degenerated primers to HVR2 region of all flax CesA genes were developed basing on comparison of six CesA EST sequences of flax and full cDNA sequences of Arabidopsis, poplar, maize and cotton plants, obtained from GenBank. After amplification of flax cDNA, the bands of expected size were detected (201 and 300 b.p. for the CesA-1 and CesA-6, and 600 b.p. for the HVR2 region of CesA respectively). The developed markers can be used for cloning and sequencing of flax CesA genes, identifying their number in flax genome, tissue and stage specificity.
PubMed: 20722279
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose synthase].</title><author><name sortKey="Grushetskaia, Z E" sort="Grushetskaia, Z E" uniqKey="Grushetskaia Z" first="Z E" last="Grushetskaia">Z E Grushetskaia</name></author><author><name sortKey="Lemesh, V A" sort="Lemesh, V A" uniqKey="Lemesh V" first="V A" last="Lemesh">V A Lemesh</name></author><author><name sortKey="Khotyleva, L V" sort="Khotyleva, L V" uniqKey="Khotyleva L" first="L V" last="Khotyleva">L V Khotyleva</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010 Jul-Aug</date><idno type="RBID">pubmed:20722279</idno><idno type="pmid">20722279</idno><idno type="wicri:Area/Main/Corpus">003096</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003096</idno><idno 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scheme="KwdEn" xml:lang="en"><term>Arabidopsis (enzymology)</term><term>Arabidopsis (genetics)</term><term>Base Sequence (MeSH)</term><term>Cell Wall (genetics)</term><term>Cell Wall (metabolism)</term><term>Cellulose (biosynthesis)</term><term>Cellulose (genetics)</term><term>DNA Primers (genetics)</term><term>DNA, Complementary (genetics)</term><term>Expressed Sequence Tags (MeSH)</term><term>Flax (enzymology)</term><term>Flax (genetics)</term><term>Flax (growth & development)</term><term>Genes, Plant (MeSH)</term><term>Glucosyltransferases (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Amorces ADN (génétique)</term><term>Arabidopsis (enzymologie)</term><term>Arabidopsis (génétique)</term><term>Cellulose (biosynthèse)</term><term>Cellulose (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glucosyltransferases (génétique)</term><term>Gènes de plante (MeSH)</term><term>Lin (croissance et développement)</term><term>Lin (enzymologie)</term><term>Lin (génétique)</term><term>Paroi cellulaire (génétique)</term><term>Paroi cellulaire (métabolisme)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Étiquettes de séquences exprimées (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Lin</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term><term>Lin</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term><term>Flax</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Cell Wall</term><term>Cellulose</term><term>DNA Primers</term><term>DNA, Complementary</term><term>Flax</term><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Flax</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Amorces ADN</term><term>Arabidopsis</term><term>Cellulose</term><term>Glucosyltransferases</term><term>Lin</term><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Expressed Sequence Tags</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Gènes de plante</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term><term>Étiquettes de séquences exprimées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cellulose synthase catalytic subunit genes, CesA, have been discovered in several higher plant species, and it has been shown that the CesA gene family has multiple members. HVR2 fragment of these genes determine the class specificity of the CESA protein and its participation in the primary or secondary cell wall synthesis. The aim of this study was development of specific and degenerated primers to flax CesA gene fragments leading to obtaining the class specific HVR2 region of the gene. Two pairs of specific primers to the certain fragments of CesA-1 and CesA-6 genes and one pair of degenerated primers to HVR2 region of all flax CesA genes were developed basing on comparison of six CesA EST sequences of flax and full cDNA sequences of Arabidopsis, poplar, maize and cotton plants, obtained from GenBank. After amplification of flax cDNA, the bands of expected size were detected (201 and 300 b.p. for the CesA-1 and CesA-6, and 600 b.p. for the HVR2 region of CesA respectively). The developed markers can be used for cloning and sequencing of flax CesA genes, identifying their number in flax genome, tissue and stage specificity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20722279</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2010</Year><Month>08</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0564-3783</ISSN><JournalIssue CitedMedium="Print"><Volume>44</Volume><Issue>4</Issue><PubDate><MedlineDate>2010 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>TSitologiia i genetika</Title><ISOAbbreviation>Tsitol Genet</ISOAbbreviation></Journal><ArticleTitle>[Development of specific and degenerated primers to CesA genes encoding flax (Linum usitatissimum L.) cellulose synthase].</ArticleTitle><Pagination><MedlinePgn>3-8</MedlinePgn></Pagination><Abstract><AbstractText>Cellulose synthase catalytic subunit genes, CesA, have been discovered in several higher plant species, and it has been shown that the CesA gene family has multiple members. HVR2 fragment of these genes determine the class specificity of the CESA protein and its participation in the primary or secondary cell wall synthesis. The aim of this study was development of specific and degenerated primers to flax CesA gene fragments leading to obtaining the class specific HVR2 region of the gene. Two pairs of specific primers to the certain fragments of CesA-1 and CesA-6 genes and one pair of degenerated primers to HVR2 region of all flax CesA genes were developed basing on comparison of six CesA EST sequences of flax and full cDNA sequences of Arabidopsis, poplar, maize and cotton plants, obtained from GenBank. After amplification of flax cDNA, the bands of expected size were detected (201 and 300 b.p. for the CesA-1 and CesA-6, and 600 b.p. for the HVR2 region of CesA respectively). The developed markers can be used for cloning and sequencing of flax CesA genes, identifying their number in flax genome, tissue and stage specificity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Grushetskaia</LastName><ForeName>Z E</ForeName><Initials>ZE</Initials></Author><Author ValidYN="Y"><LastName>Lemesh</LastName><ForeName>V A</ForeName><Initials>VA</Initials></Author><Author ValidYN="Y"><LastName>Khotyleva</LastName><ForeName>L V</ForeName><Initials>LV</Initials></Author></AuthorList><Language>rus</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Ukraine</Country><MedlineTA>Tsitol Genet</MedlineTA><NlmUniqueID>0101671</NlmUniqueID><ISSNLinking>0564-3783</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019597" MajorTopicYN="N">Flax</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>8</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>8</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20722279</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Grushetskaia, Z E" sort="Grushetskaia, Z E" uniqKey="Grushetskaia Z" first="Z E" last="Grushetskaia">Z E Grushetskaia</name><name sortKey="Khotyleva, L V" sort="Khotyleva, L V" uniqKey="Khotyleva L" first="L V" last="Khotyleva">L V Khotyleva</name><name sortKey="Lemesh, V A" sort="Lemesh, V A" uniqKey="Lemesh V" first="V A" last="Lemesh">V A Lemesh</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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